PCR

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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sarah111
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PCR

Post by sarah111 » Sat Mar 12, 2011 12:37 am

Hi im abit confussed and would really appreciate if someone could help me with this.
The project objective is to obtain actin gene from genomic DNA, insert amplicon into a plasmid and confirm with qRTPCR.
How exactly would i go about doing this?

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JackBean
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Post by JackBean » Sat Mar 12, 2011 8:54 am

find your gene e.g. at PubMed or some other specialized database (depends on your organism), then you need to design primers for that sequence, best with some restriction places. Cut the amplicon and vector with RE. Mix and ligate.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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