Reverse transcription (hexamers, decamers or oligo d(T))

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joann2717
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Reverse transcription (hexamers, decamers or oligo d(T))

Post by joann2717 » Thu Mar 10, 2011 3:16 pm

Hi

I am currently looking into reverse transcription techniques. I am using the MMLV reverse transcriptase kit but wanted to know which primer is best to use. I am unsure of the differences between random hexamers, decamers and oligo d(T) primers. I have isolated mRNA prior to the RT instead of total RNA and this is what i use in the reaction. The down stream reactions will be real-time PCR and end point PCR (for mRNA specific markers) and possibly fragment analysis.

Any help would be greatly appreciated.

Thanks

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