Devising Experiments to Determine Positive Regulation

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Devising Experiments to Determine Positive Regulation

Post by niravana21 » Mon Mar 07, 2011 5:49 am

Hey members, I was having difficulity with this biology problem. I've included a picture, to avoid confusion.

For a. i would use DNA footprinting to confirm that A and B bind to the DNA whereas C would not.

However, I don't know what type of experiment I would use to determine the circumstances in B and C?

By experiment I mean, EMSA, Northern / Southern Blotting, DNase Sensitivity assay etc.

Any ideas?

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Inland Taipan
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Post by JackBean » Mon Mar 07, 2011 7:03 am

you could knock-down/overexpress your proteins (A,B or C) and see, what does the level of the regulated gene X

Cis or trans? That's what matters.

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