ribosome stall

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taotaopei
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ribosome stall

Post by taotaopei » Thu Aug 18, 2005 5:56 pm

If I anneal a DNA oligo to the end of a mRNA strand, then perform in vitro translation. My question is can this double helix stall ribosome or not? Thanks.

sdekivit
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Post by sdekivit » Thu Aug 18, 2005 6:45 pm

depends on where you put it. If you have a mature mRNA and after it you put the DNA, nothing will happen, because the stopsignal is in front of the poly-A-tail and the 3' UTR of the mRNA.

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Post by taotaopei » Thu Aug 18, 2005 7:27 pm

The DNA oligo is complementary to the mRNA before the stop codon. So, what will happen. Is there any pape about this?

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Post by sdekivit » Fri Aug 19, 2005 4:28 pm

taotaopei wrote:The DNA oligo is complementary to the mRNA before the stop codon. So, what will happen. Is there any pape about this?


i think that the translation will be stopped, bacause there's an obstacle for the ribosome. Eeventually it will fall of. But this is not based on facts, just my guess.

I don't know if this is tried before in an experiment.

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