active and allosteric sites

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briteyellowness
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active and allosteric sites

Post by briteyellowness » Thu Aug 18, 2005 1:14 pm

the description says:

in one patient with recurrent gout, excreted uric acid levels were found to be three times normal levels. the patient's red blood cells exhibited markedly increased levels of 5-PRPP. Assays revealed that the patient had normal levels of PRPP synthase, but the enzyme activity was three times normal levels in cultured cells. The pH optimum and enzyme activity of the purified enzyme were normal.

how do i know that the increased levels of uric acid is a mutation that affects the allosteric site of PRPP synthetase and not the active site?

sdekivit
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Post by sdekivit » Thu Aug 18, 2005 3:56 pm

if there was an enhancement in the active site, the mutation must be in the gene for the enzyme. Since the enzyme is just normal (when purified the effect is normal), the effect must be allosteric.

When the active site of the enzyme is affected, the purified enzyme would also have an increased activity.

briteyellowness
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Post by briteyellowness » Thu Aug 18, 2005 4:08 pm

what does it mean when the enzyme was purified?

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mith
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Post by mith » Thu Aug 18, 2005 9:57 pm

separated from the mix.
Living one day at a time;
Enjoying one moment at a time;
Accepting hardships as the pathway to peace;
~Niebuhr

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