Single stranded DNA

Genetics as it applies to evolution, molecular biology, and medical aspects.

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vinodpaul
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Single stranded DNA

Post by vinodpaul » Tue Feb 22, 2011 10:24 am

Hi Can anyone tell me some of the commercially available single stranded DNA. I searched in internet and i found only M13mp18 which is 7250 bp. I need DNA which has around 2000 bases.
Thank You
Last edited by vinodpaul on Tue Feb 22, 2011 2:41 pm, edited 1 time in total.

vinodpaul
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Post by vinodpaul » Tue Feb 22, 2011 2:39 pm

or can some one please tell me how can i cut this m13mp18 dna to a 2000 bases long dna

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JackBean
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Post by JackBean » Tue Feb 22, 2011 5:54 pm

probably by some nuclease. Maybe keep it with exonuclease for some time...

Or you could try to digest normal DNA and then do some affinity chromatography specific for only one strand. But I don't know, whether you could get only one strand :(
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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Post by vinodpaul » Wed Feb 23, 2011 1:49 am

_at_ JackBean Thank you for your reply ... Do you know about any commercially available single stranded DNAs

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JackBean
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Post by JackBean » Wed Feb 23, 2011 9:11 am

no sorry, I have no idea
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

angel92
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Post by angel92 » Thu Mar 17, 2011 6:18 pm

i think so that restriction enzymes cut the strand into fragments :P

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