Plotting glucose production against time

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SWEngineer
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Plotting glucose production against time

Post by SWEngineer » Sat Feb 05, 2011 2:14 pm

Working on a pre-lab, some of the directions for drawing graphs is that the "x-axis" is for the non-variables, and the "y-axis" is for the variables (what you have measured).

Now, the first thing I have to do is to make a standard curve for "Glucose concentration" and "Absorbtion".

The next step is to measure the "Enzyme activity" and use the standard curve done to deternmine the amount of glucose since I will be having the "Absorbance" values in the "Enzyme activity" process.

Finally, we are asked to plot a graph that shows the glucose production against time.

Based on that, what (glucose production, time) goes where (x-axis, y-axis)?

Thanks.

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JackBean
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Post by JackBean » Sat Feb 05, 2011 5:25 pm

first, you need to make the standard curve of absorbance (y) dependent on Glc conc (x). Make linear regresion and from that formula you will calculate amount of produced/consumed Glc.
That amount you will re-calculate to the enzymatic activity and that will you plot on y-axis and time on x-axis
http://www.biolib.cz/en/main/

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SWEngineer
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Re:

Post by SWEngineer » Sun Feb 06, 2011 7:39 pm

JackBean wrote:first, you need to make the standard curve of absorbance (y) dependent on Glc conc (x). Make linear regresion and from that formula you will calculate amount of produced/consumed Glc.
That amount you will re-calculate to the enzymatic activity and that will you plot on y-axis and time on x-axis


Thanks so much for your reply.

When you say: "Make linear regresion and from that formula you will calculate amount of produced/consumed Glc."

Do you mean using the linear line formula: y = mx + c ?

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JackBean
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Post by JackBean » Sun Feb 06, 2011 8:00 pm

yes, exactly
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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