## Plotting glucose production against time

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SWEngineer
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### Plotting glucose production against time

Working on a pre-lab, some of the directions for drawing graphs is that the "x-axis" is for the non-variables, and the "y-axis" is for the variables (what you have measured).

Now, the first thing I have to do is to make a standard curve for "Glucose concentration" and "Absorbtion".

The next step is to measure the "Enzyme activity" and use the standard curve done to deternmine the amount of glucose since I will be having the "Absorbance" values in the "Enzyme activity" process.

Finally, we are asked to plot a graph that shows the glucose production against time.

Based on that, what (glucose production, time) goes where (x-axis, y-axis)?

Thanks.

JackBean
Inland Taipan
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Joined: Mon Sep 14, 2009 7:12 pm
first, you need to make the standard curve of absorbance (y) dependent on Glc conc (x). Make linear regresion and from that formula you will calculate amount of produced/consumed Glc.
That amount you will re-calculate to the enzymatic activity and that will you plot on y-axis and time on x-axis
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

SWEngineer
Garter
Posts: 34
Joined: Sat May 08, 2010 1:24 pm

### Re:

JackBean wrote:first, you need to make the standard curve of absorbance (y) dependent on Glc conc (x). Make linear regresion and from that formula you will calculate amount of produced/consumed Glc.
That amount you will re-calculate to the enzymatic activity and that will you plot on y-axis and time on x-axis

When you say: "Make linear regresion and from that formula you will calculate amount of produced/consumed Glc."

Do you mean using the linear line formula: y = mx + c ?

JackBean
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm
yes, exactly
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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