Differentially Label

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chengieteo
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Differentially Label

Post by chengieteo » Sat Feb 05, 2011 3:19 am

Hi all,

I'm quite new in Molecular biology and wish to know more. I saw this two question not very sure how we can do it.

How can we differentially label (make different) DNA and RNA?
How can we differentially label (make different) DNA/RNA and protein?

Hope that someone can assist me in this. Thanks in advance =)

Regards,
Cheng

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JackBean
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Post by JackBean » Sat Feb 05, 2011 10:01 am

for labeling NAs you can use e.g. Cy3 and Cy5 or plenty fluorescent colors used in qPCR such as TaqMan and many others.

And I think that Cy3 and Cy5 can be used even for proteins. For some other labels look for differential 2D electrophoresis.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

peyem
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Re: Differentially Label

Post by peyem » Tue Feb 08, 2011 5:36 am

For RNA and DNA, is it the uracil in RNA and the thymine in DNA that will produce different fluorescent colours?

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canalon
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Post by canalon » Wed Feb 09, 2011 2:29 am

There are countless fluorescent markers who have different physical properties and are able to give different colors.
T and U are not fluorescent by themselves, but if a fluorescent moiety is attached to them (obviously with different spectral properties) they will be easy to separate.

Handy list of different fluorophores here:
http://en.wikipedia.org/wiki/Fluorophore

One thing they have in common though is that they are quite expensive.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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