Standard curve - Concentration

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SWEngineer
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Standard curve - Concentration

Post by SWEngineer » Fri Feb 04, 2011 11:45 am

I just want to ask about calculating the concentration part of the standard curve.

Say I have the following tubes with the mentioned data:

Tube (A):
Glucose (5mg/ml) = 0ml
Water = 5ml

Tube (B):
Glucose (5mg/ml) = 1ml
Water = 4ml

Tube (C):
Glucose (5mg/ml) = 2ml
Water = 3ml

Now, when I want to plot a standard curve, what should the values for the concentration be (x-axis)?

Thanks.

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JackBean
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Post by JackBean » Fri Feb 04, 2011 4:15 pm

simply calculate by c1*V1 = c2*V2
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

SWEngineer
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Re:

Post by SWEngineer » Fri Feb 04, 2011 6:15 pm

JackBean wrote:simply calculate by c1*V1 = c2*V2


I found the solution in another way, but this formula I think makes more sense.

Thanks.

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JackBean
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Post by JackBean » Fri Feb 04, 2011 7:12 pm

good for you
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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