Molecular Genetics-Mapping

Genetics as it applies to evolution, molecular biology, and medical aspects.

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prenurse
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Molecular Genetics-Mapping

Post by prenurse » Thu Feb 03, 2011 12:49 am

PRIMER EXTENSION QUESTIONS:

1. Why does primer extension NOT work if one is trying to find the 3’ end of a mRNA transcript?


2. If you wanted to determine a transcription start site down to the very first base, when procedure would have to carry out AFTER doing primer extension?to



1. What does the Greek letter γ refer to and why is [γ-32P] ATP used?


2.In step “b”, why is [α-32P]NTP used? Specifically, what does α refer to and why is NTP rather than ATP listed?

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JackBean
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Post by JackBean » Thu Feb 03, 2011 7:13 am

1. think about in what direction goes the DNA Pol ;)

2. you want to determine the sequence of your mRNA. How is that process called? Read about it's limitations ;)

1. do you know, what does ATP stand for? If you don't tell us, in what process, we won't tell you (although I have a guess)

2. the same as in 1.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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