Top-down and bottom-up technology

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u05sp0
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Top-down and bottom-up technology

Post by u05sp0 » Thu Jan 20, 2011 7:29 pm

Hi there,

I was wondering if anybody is able to explain top-down and bottom-up technology in terms of DNA sequencing? I have no clue how it works and I have minimal notes on it!

Thanks :)

Seren

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JackBean
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Post by JackBean » Thu Jan 20, 2011 7:33 pm

I would guess it's like this:
top-down - you first sort your chromosomes, then make from them smaller and smaller pieces (but you somehow know the order) and these pieces you sequence
bottom-up - you just break all your DNA, sequence (preferentially on Next-Gen sequencing machines) and from these small pieces you assemble the whole genome.

But I'm just guessing, I have never heared it in relation to DNA sequencing
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

u05sp0
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Re: Top-down and bottom-up technology

Post by u05sp0 » Thu Jan 20, 2011 8:01 pm

Thanks :) I'm not sure if that's how it goes, but I don't know myself either so I can't really say! I'm trying to look in my text book for it but no luck so far :?

Seren x

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