Renaturing/re-annealing denatured DNA strands?
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Renaturing/re-annealing denatured DNA strands?
Hello,
I am having difficulty answering the following question:
When the temperature is lowered and the original denatured DNA strands can reanneal, or renature, in addition to the full double-stranded molecules, some molecules of the type shown in the image attached are seen when the molecules are examined under the electron microscope. How can you explain these structures?
http://img267.imageshack.us/i/diagram1r.png/
any help would be greatly appreciated!
thanks
I am having difficulty answering the following question:
When the temperature is lowered and the original denatured DNA strands can reanneal, or renature, in addition to the full double-stranded molecules, some molecules of the type shown in the image attached are seen when the molecules are examined under the electron microscope. How can you explain these structures?
http://img267.imageshack.us/i/diagram1r.png/
any help would be greatly appreciated!
thanks
Re: Renaturing/re-annealing denatured DNA strands?
thank you JackBean for the previous answer and this answer but I think they are asking how do these "circular" structures that you can see in the picture I attached are formed? Maybe due to mistakes in complementary base pairing when the strands re-anneal?
Re: Renaturing/re-annealing denatured DNA strands?
makes sense! thank you very much:)
By the way those circular structures, or more exactly bubbles are usually known as hairpin structures. They can be a problem when you are designing primers. They are also essential for example to the correct folding and activity of ribozymes, tRNA and of the ribosome, and they are also used to regulate the translation of some mRNA.
And your prof has master skillz with his electron microscope if he can see this kind of things. They are more commonly demonstrated with some nuclease assays.
And your prof has master skillz with his electron microscope if he can see this kind of things. They are more commonly demonstrated with some nuclease assays.
Patrick
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
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