Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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When trying to plot binding curves such as competitive binding curves, is the non-specific binding (NSB) sample required?
- Inland Taipan
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- Joined: Mon Sep 14, 2009 7:12 pm
I think it's good, because you can say "OK, if there is nonspecific binding e.g. by albumin (or whatever), it's just 3%, while with the same amount of my protein it is 20%, so it's probably specific"
Cis or trans? That's what matters.
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