PCR cDNA

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
magicsiew
Coral
Coral
Posts: 145
Joined: Sun Jan 24, 2010 3:07 pm

PCR cDNA

Post by magicsiew » Tue Dec 21, 2010 2:33 pm

Hi, in order to save cost of RT-PCR, I was thinking to do reverse transcription from mRNA to cDNA and use that cDNA in PCR for large scale screening purpose. I would like to ask will these two reaction give same results? Both RT-PCR using RNA and PCR using cDNA. Thank you.

Nicky2010
Garter
Garter
Posts: 2
Joined: Tue Dec 14, 2010 4:05 am

Post by Nicky2010 » Thu Dec 23, 2010 6:13 am

Hi
I think these two reaction can give the same results.
Because RT-PCR refers to reverse transcription PCR and include "mRNA to cDNA" and "c
DNA to dsDNA.
In fact, doing reverse transcription from mRNA to cDNA yourself is one step of RT-PCR. they are the same.

Post Reply

Who is online

Users browsing this forum: Bing [Bot] and 9 guests