Virus protein coat

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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snow88
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Virus protein coat

Post by snow88 » Fri Dec 17, 2010 11:29 pm

I am currently dealing with IHHNV virus in M. rosenbergii. My task is to clone the capsid gene of IHHNV. However, i am facing problem with getting the capsid gene out from the genome. The primers i used are designed from Peneaus spc. May i know how often does virus protein coat undergo mutation? Is it feasible if i use protein coat sequence from other related species to design primers for my species?

magicsiew
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Post by magicsiew » Sat Dec 18, 2010 8:16 am

Yes, you may do that, try to collect as much sequences as possible from related species of your particular gene and find the conserved region.

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Post by magicsiew » Sat Dec 18, 2010 11:40 am

http://www.ncbi.nlm.nih.gov/pmc/article ... 011799.pdf

Seems that the mutation can occur in a relatively high rate.

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