Why cytospin?

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Why cytospin?

Post by evilid » Tue Dec 14, 2010 2:51 am

I would like to some Immunocytochemistry.
I have seen people prepare their suspension cells (fix, permeabilize, block, stain), then put on sticky slides (either by adhesive slides or poly-L-Lysine coated plate) with a mounting medium.
But some people cytospin their cells on a slide and process them after that.

So I am just wondering what is rational behind of the use of cytospin for cultured cells?

Thank you.

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