Is blood good for DNA fingerprinting?

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EmmVeePee
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Is blood good for DNA fingerprinting?

Post by EmmVeePee » Sat Aug 13, 2005 8:07 am

I recall that red blood cells lose many of their chromosomes .

So would blood be a poor sample for DNA fingerprinting, or do they use a specific nuclease?

Corrent me if I'm retarded.
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Post by Dr.Stein » Sat Aug 13, 2005 9:22 am

Yeah, that's a big question mark in my head since human RBC doesn't have nucleus :?
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Post by EmmVeePee » Sat Aug 13, 2005 6:28 pm

I'm guessing that other cells in the blood are used.
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Post by MrMistery » Sat Aug 13, 2005 8:23 pm

Correct. While adult red blood cells lose their nucleus, thus making them useless for anything requiring DNA, leucocytes still contain the entire human genome, so they are used for DNA fingerprinting
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Post by mith » Sat Aug 13, 2005 10:05 pm

What would you suggest we take cells from instead? Cheek cells or skin cells?

There might be some problems with contamination.
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Post by EmmVeePee » Sun Aug 14, 2005 1:18 am

mithrilhack wrote:What would you suggest we take cells from instead? Cheek cells or skin cells?

There might be some problems with contamination.


Precisely.

How much blood is needed to obtain a sufficient sample?
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Post by Jelanen » Sun Aug 14, 2005 1:29 am

Well, DNA can be obtained from a cheek swab also. And, since its run through PCR, you don't need a whole lot..
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Post by EmmVeePee » Sun Aug 14, 2005 6:45 am

I wasn't sure how much blood was needed for PCR.

So a drop of blood would be more than sufficient, correct? (Hell, if a swab is sufficient..)
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Post by mith » Sun Aug 14, 2005 12:57 pm

anything without needles would be less traumatizing :D
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Post by MrMistery » Sun Aug 14, 2005 7:45 pm

If you were lucky enough to find one white blood cell which you can extract clean DNA from, that wil be enough. However, one cell remaining intact in the outside world would be virtually impossible
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Post by th1_rhs13 » Mon Aug 15, 2005 4:48 am

Gametes!

mithrilhack wrote:What would you suggest we take cells from instead? Cheek cells or skin cells?

There might be some problems with contamination.


What exactly is neccesary to obtain your objective?

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Post by MrMistery » Mon Aug 15, 2005 8:44 pm

th1_rhs13 wrote:Gametes!

I am not sure gamets would be a good option since they only contain half of the DNA strain
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