Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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Hello everyone..I wonder if someone could help me find the optimum molar insert:vector ratio (or ratio range) to clone my gene into the pET28a(+) vector. If there is anyone who used this expression vector before, I would appreciate any recommendation. Thanks in advance..
Kezzer wrote:Hey, I used PET28c using a molar ration of 1:8 if 1:3 isn't suitable maybe try that
what a coincidence I just tried 1:7 and 1:3 ratios and performed transformation, so I'll get the results in a couple of days. Thank you..
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