Identifying an unknown

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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Identifying an unknown

Post by SurrealFantasy » Thu Nov 25, 2010 2:21 am

Hi, I am currently taking my microbiology lab final and as is with most microbiology classes, the final consists of identifying an unknown. In my case, we were all given a different test tube which contains two different bacteria. I was hoping to find some help from you all.
So far, I have done a gram's stain, acid-fast stain and a spore stain.
The gram's stain concluded many purple rod-shaped cells, which would lead me to believe that it is gram-positive.
The acid-fast stain concluded both pink and purple colors
The spore stain did not yield any results.

On top of the stains, I have done dishes of a variety of media.
MSA: I attached a picture of this to my post, as it had the most growth. In case you can't tell, it is white, flaky, and milky looking.
TSA: Yellow colones
NA: White colonies
EMB: No growth at all

The possible bacteria that we can be given are: Bacillus subtillis, Staph aureus, Mycobacterium Smegmatis, Micrococcus Luteus, Proteus Vulgaris, Serratia mercescens, Pseudomonas aeruginosa, Escherichia coli
Also, Staph and micrococcus are not a possible combination.

I'm fairly certain that one of them is Mycobacterium Smegmatis.

I really appreciate the help!

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