SONICATION OF DNA

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

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bill1
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SONICATION OF DNA

Post by bill1 » Wed Nov 17, 2010 11:19 pm

Heya,

Could you help me with this subject? I need to find out how long do I need to sonicate DNA? any suggestions any publications??

I really appreciate your help :)

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canalon
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Post by canalon » Thu Nov 18, 2010 1:11 am

Have you tried Google? lots of protocols available for free.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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JackBean
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Post by JackBean » Thu Nov 18, 2010 6:51 am

why do you want to sonicate DNA?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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canalon
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Post by canalon » Thu Nov 18, 2010 1:56 pm

To shear it in small and relatively constant fragment before say cloning. You can also use a fine needle or a nebulizer. it is cheaper and less sequence specific than restriction enzyme, with break point that should be entirely random, which should give a better chance to get complete coverage for sequence, or to avoid cutting in the middle of your gene of interest.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

bill1
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Post by bill1 » Wed Nov 24, 2010 7:47 pm

Thank you for all replies. Unfortunately I wasn't able to find as many protocols about sonication as I want:(
If anyone of you has knowledge or links about it I would really appreciate it.

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canalon
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Post by canalon » Wed Nov 24, 2010 7:54 pm

Things like:
http://www.kreatech.com/Default.aspx?tabid=119
http://www.genome.ou.edu/protocol_book/ ... artII.html

First and 5th link in google for DNA sonication. I fail to see what more you would need.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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