Removing DNA contaminations with Sodium hypochlorite

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TechAssistantFTW
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Removing DNA contaminations with Sodium hypochlorite

Post by TechAssistantFTW » Tue Nov 16, 2010 11:15 am

To decontaminate my bench before doing PCR I'm following this advice here:

http://bergman-net.com/Service_web/Remo ... 0DNA_1.pdf

It suggests using a 5-10% solution of Sodium hypochlorite so I bought a bottle of this:

http://uk.vwr.com/app/TechnicalDataSheet?catnum=1385932

It is labelled "Sodium hypochlorite ± 3,5 % active" and I thought it's a 3,5% solution of NaOCl, but I guess the 3,5% means something else? What exactly and can I use it for decontamination?

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canalon
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Re: Removing DNA contaminations with Sodium hypochlorite

Post by canalon » Tue Nov 16, 2010 2:34 pm

TechAssistantFTW wrote:To decontaminate my bench before doing PCR I'm following this advice here:

http://bergman-net.com/Service_web/Remo ... 0DNA_1.pdf

It suggests using a 5-10% solution of Sodium hypochlorite so I bought a bottle of this:

http://uk.vwr.com/app/TechnicalDataSheet?catnum=1385932

It is labelled "Sodium hypochlorite ± 3,5 % active" and I thought it's a 3,5% solution of NaOCl, but I guess the 3,5% means something else? What exactly and can I use it for decontamination?

It is labeled "Sodium hypochlorite ± 3,5 % active Cl" The active Cl is the disinfecting part of the NaOCl solution. This is what you nedd, and it appears that what you bought is slightly less concentrated that what is recommended in the protocol you linked to. But I guess you could just go to the nearest shopping mall and buy a bottle of no name thin bleach (hypochlorite based of course) should have the percentage of active chlorine somewhere on the label.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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JackBean
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Post by JackBean » Wed Nov 24, 2010 9:39 am

hypochlorite quite smells, I think it's more convenient to use simply EtOH ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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