Suspension cell

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bgs
Garter
Garter
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Suspension cell

Post by bgs » Fri Nov 12, 2010 4:15 am

Dear users,

We are starting to do cell culturing of suspension cells. Wondering if there are techniques to see viable cells other than tryphan blue and how to separate dead and living cells.

Regards
bgs

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canalon
Inland Taipan
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Post by canalon » Fri Nov 12, 2010 5:40 pm

FACS and live/dead cell fluorescent dyes (like propidium iodide) It is quick and easy if you have acess to this kind of machine. Otherwise no idea.
Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

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