Determining gene order of cotransfers?

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Determining gene order of cotransfers?

Post by canucksftw » Tue Nov 09, 2010 11:10 pm

How do I do number 21 B? How can one determine the order of the genes by just looking at the percentages? What do I need to do? Thanks

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Post by canalon » Wed Nov 10, 2010 12:10 am

Basically the problem is that if you have 3 genes ABC (in that order) that can be excised during by the phage the frequency of transformants with either A or C are high, AB and BC are less frequent because you need to cut a larger fragment out and AC (or CA) very low. The closer the genes, the higher the frequency of co-transduction.

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Post by Julie5 » Wed Nov 10, 2010 8:26 am

"The closer the genes, the higher the frequency of co-transduction."

Just checking - but does this mean that the closer the genes are, the harder they are to separate, so they are more likely to be transferred together than genes which are further apart?

What's the closest together that genes can ever be? Are they ever contiguous (allowing for initiation/stop codons etc)?

(Sorry to ask a basic question - I'm still getting my head round all this gene mullarky!)(it's all changed SOOOOO much since my day - a long time ago!)

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Post by JackBean » Wed Nov 10, 2010 3:35 pm

the border of transformed DNA can be virtually anywhere, so if are the genes close, there is smaller chance, that the border will be just between them.

Look for Rhodococcus fascians and his virulence plasmid :)

Cis or trans? That's what matters.

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