Breaking outer membranes of red blood cells

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brspurri
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Breaking outer membranes of red blood cells

Post by brspurri » Wed Aug 10, 2005 7:02 pm

Hi,

I am trying to figure out what pressure difference (outside cell membrane to inside cell membrane) will rupture a red blood cell. I was thinking I need to figure out the tension of the membrane and then just perform some simle calculations, but I am not sure how to even get this. Can anyone help? If it is easier, you can find me on AIM at UCDLightweight or Yahoo at Brett_s20.

Thanks,
Brett

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Dr.Stein
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Post by Dr.Stein » Thu Aug 11, 2005 4:05 am

I am not good at calculation, but I can tell you how to break RBC's cell membrane, i.e. by applying with 0.1 N HCl. This method is common in measuring haemoglobin level.
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sdekivit
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Post by sdekivit » Thu Aug 11, 2005 4:11 pm

Dr.Stein wrote:I am not good at calculation, but I can tell you how to break RBC's cell membrane, i.e. by applying with 0.1 N HCl. This method is common in measuring haemoglobin level.


you can also put some blood in distillated water. Due to osmosis, the RBC will go to lysis.

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