Poly A >>> what the propose ?

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Nmr
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Poly A >>> what the propose ?

Post by Nmr » Sun Oct 31, 2010 7:52 am

Hi guys i need ur help >>>

what the propose of adding the poly A tail to the 5' terminus of the primer in PCR in Megaprimer method ?

thanx

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JackBean
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Post by JackBean » Sun Oct 31, 2010 5:00 pm

do you have somewhere some protocol or principle? I was looking on Google, but there are only original articles...
http://www.biolib.cz/en/main/

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Nmr
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Post by Nmr » Mon Nov 01, 2010 4:02 am

Actually.. i do by Megaprimer... using certain protocol... and as i know it's better to add poly A tail, but the problem is my lecturer said that no need to add poly A tail.?! so now i got confuse about the function of Poly A tail and what the propose of adding it ??!!

pleas if anyone know about that, can he/she give a brief explanation about the poly A function?

thanx

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JackBean
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Post by JackBean » Mon Nov 01, 2010 7:42 am

I thought, whether could you provide us with some protocol?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Nmr
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Post by Nmr » Mon Nov 01, 2010 9:08 am

http://www.protocol-online.org/prot/

here u can get whatever protocol u want...

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JackBean
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Post by JackBean » Mon Nov 01, 2010 10:00 am

did not find
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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