cDNA hybridization

Genetics as it applies to evolution, molecular biology, and medical aspects.

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june320
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cDNA hybridization

Post by june320 » Fri Oct 29, 2010 1:24 pm

the cDNA produced using mature mRNA is not exactly complementary to the gene sequence, right? jst wondering how does the cDNA hybridize with the DNA? is it that only part of the cDNA hybridize with the complementary DNA sequence?

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JackBean
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Post by JackBean » Fri Oct 29, 2010 2:18 pm

with which DNA?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

june320
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Post by june320 » Fri Oct 29, 2010 2:32 pm

DNA from the cell, for example in mRNA microarray

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JackBean
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Post by JackBean » Fri Oct 29, 2010 9:39 pm

in microarray you use usually short pieces of DNA, which are supposed to be complementary, so you arrange them to the exons of course
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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