Can normal cells survive without changing medium?

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eTiT
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Can normal cells survive without changing medium?

Post by eTiT » Wed Aug 10, 2005 1:30 am

Why are normal cells able to survive longer than transformed or continuous cell lines at high density without changing the medium? Isn't lack of medium will cause the normal cells to die?

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chemistry_freako
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Post by chemistry_freako » Wed Aug 10, 2005 3:57 pm

You mean normal media, or?
化学は、最高です

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Dr.Stein
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Post by Dr.Stein » Thu Aug 11, 2005 3:42 am

I think you should still renew the medium of your cell culture after some period.
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Post by DevGrp » Mon Aug 22, 2005 9:04 am

Depends on the cells but in my exprience normal cells (in my case HUVEC / breast / endometrial primary cells) are far more sensitive to old media / high density than transformed cell lines.
If you don't change HUVEC media every two days or don't split them before confluence you soon have lots of dead cells

Also of concern is that if you are using Pen/strep antibiotics in your media then this isn't stable for very long at 37C (a few days?) so bacterial contamination can be a problem with old media

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