Buffer solutions

Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
sanzie
Garter
Garter
Posts: 1
Joined: Mon Oct 25, 2010 1:22 am

Buffer solutions

Post by sanzie » Mon Oct 25, 2010 1:33 am

Hi can anyonehelp me solve this, I know it should be easy but I can't work it out

Given 1M of acetic acid
and 1M NaOH
You are to prepare l litre of a 0.15m acetate buffer pH is 5.0 and pKa is 4.7

1.How many mLs of hte 1M acetic acid will you use?

2.In the final buffer solution what is the concentration of acetic acid + acetate ion

3.what is the ratin of acetic acid to acetate ion

4.how much of the 1M Noah will you add to make this buffer

Thanks

User avatar
JackBean
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Thu Oct 28, 2010 2:58 pm

1) use c1V1 = c2V2

2) calculate by second calculation in here http://en.wikipedia.org/wiki/Buffer_sol ... n_constant

3) that's the ratio in previous equation

4) equals to the amount of acetate
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Post Reply

Who is online

Users browsing this forum: No registered users and 4 guests