Discussion of all aspects of biological molecules, biochemical processes and laboratory procedures in the field.
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Most people use Ethidium Bromide (EtBr) as a fluorescent dye for DNA. People know it is poisonous and dangerous to cause cancer. Also people use UV to excite EtBr, which is risky for eyes and skins. Some people start to use safe dyes, like SyBr Safe, Gelgreen, Gelred, etc. They are safer but more expensive than EtBr, and can be excited with visible light which is much safer than UV. There are LED Transilluminators, such as UltraBright LED Transilluminator (Maestrogen), SafeImager (Invitrogen), and Dark Reader (Clare), that can be used as a benchtop instrument for these safe dyes well. Our lab is still using Etbr. I just wanna know what other people's opinion about using safe dyes instead. I heard in US there is almost 30% using safe dyes for DNA.
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