Anyone has any idea what is cobblestone area forming cells??

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Anyone has any idea what is cobblestone area forming cells??

Post by [email protected] » Tue Aug 09, 2005 5:21 pm

Anyone has any idea what is cobblestone area forming cells?? :cry:

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ginny
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hummm I think I have an idea ???

Post by ginny » Tue Aug 09, 2005 6:35 pm

CD34+ · CD34- · Cobblestone-area-forming cells · Cord blood · Bone marrow stroma

The long-term culture-initiating cell (LTC-IC) assay is a physiological approach to the quantitation of primitive human hematopoietic cells. The readout using identification of cobblestone area-forming cells (CAFC) has gained popularity over the LTC-IC readout where cells are subcultured in a colony-forming cell assay. However, comparing the two assays, cord blood (CB) mononuclear cell (MNC) samples were found to contain a higher frequency of CAFC than LTC-IC (126 ± 83 versus 40 ± 31 per 105 cells, p = 0.0001). Overall, 60% of week-5 cobblestones produced by CB MNC were not functional LTC-IC and were classified as "false." Separation of CB MNC using immunomagnetic columns showed that false cobblestones were CD34-/lineage+. Purified CD34+ cells, as expected, gave very similar readouts in the two assays, with 4,084 and 3,468/105 cells being CAFC and LTC-IC, respectively. CD34-/lineage- cells did not form cobblestones or become CD34+ on stroma or in cytokine culture. Human CB MNC contain a population of mature lineage+ cells, possibly mature T or B cells, which, although producing cobblestone areas (CA), are not functional LTC-IC. The CAFC readout by this method, therefore, is unreliable for estimation of primitive hematopoietic cells by limiting dilution analysis in whole human CB or MNC and also may not detect CD34- CA stem cells.




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