non-radioactive ATP analog for kinase assay?

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Joined: Tue Aug 09, 2005 7:59 am

non-radioactive ATP analog for kinase assay?

Post by unibas » Tue Aug 09, 2005 8:06 am

I am trying to check wether a certain protein is a substrate for the mTOR kinase in mammalian tissue culture (NIH 3T3 cells). I want to feed the cells with labeled ATP and then IP the protein and see if mTOR inhibition would change its phosphorylation status.
I really don't want to use such a high amount of 32P-ATP, does anyone know of a non-radioactive analog that can be used instead?

King Cobra
King Cobra
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Post by sdekivit » Tue Aug 09, 2005 3:16 pm

radioactive N or O-labelling maybe.

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