Genetics as it applies to evolution, molecular biology, and medical aspects.
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- Posts: 4
- Joined: Sat Oct 09, 2010 5:01 am
if the isolated DNA is not treated RNase, then RNA are present, but how come RNA molecules don't get separated like the digested DNA fragments? aren't they also in different sizes?
- Posts: 18
- Joined: Thu May 13, 2010 12:01 pm
They get separated like DNA, but remember how many different RNA molecules are in a cell,its not like chromosomes or fragments of restriction digests having a discrete size. Apart from this if you do not carefully prepare your samples RNAse contamination can occur quite often...
- Inland Taipan
- Posts: 5694
- Joined: Mon Sep 14, 2009 7:12 pm
as sorin wrote, there are plenty of RNAs in the cell each with different length (even the polyA can only differ)
Cis or trans? That's what matters.
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