why do RNA show smear instead of discrete bands?

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tracyliu
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why do RNA show smear instead of discrete bands?

Post by tracyliu » Sat Oct 09, 2010 11:02 pm

if the isolated DNA is not treated RNase, then RNA are present, but how come RNA molecules don't get separated like the digested DNA fragments? aren't they also in different sizes?

sorin
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Post by sorin » Sun Oct 10, 2010 8:16 am

They get separated like DNA, but remember how many different RNA molecules are in a cell,its not like chromosomes or fragments of restriction digests having a discrete size. Apart from this if you do not carefully prepare your samples RNAse contamination can occur quite often...

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JackBean
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Post by JackBean » Sun Oct 10, 2010 10:27 am

as sorin wrote, there are plenty of RNAs in the cell each with different length (even the polyA can only differ)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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