chymotrypsin mechanism

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chymotrypsin mechanism

Post by nickname » Thu Oct 07, 2010 9:25 pm

what are the correct steps for the mechanism of chymotrypsin?

Below is my answer but I don't know if it is right.

a) His 57 catalyzes removal of H from Ser 195 hydroxyl.
b. Ser 195's nucleophilic O attacks carbonyl C of substrate.
c. His 57 donates H to N of sissile peptide bond, tetrahedral intermediate decomposes.
d. C- terminal portion of oribinal substrate with its new amino acid terminus diffuses away.
e. water donates H to His 57.
f. Resulting OH attacks carbonyl of remaining substrate.
g. His 57 donates H to Ser 195 O, leading to collapse of tetrahedral intermediate.
h.N-terminal portion of original substrate with its new carboxy terminus diffuses away.

My answer is b,a,c,e,f,g,h,d. Is this right? Thank you! :)

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