Need help calculating ATP

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ayi
Garter
Posts: 2
Joined: Mon Oct 04, 2010 3:22 am

Need help calculating ATP

How do you calculate the atp in 80 carbon fat in fatty acid party of the molecule? Can someone please break it down for me?

JackBean
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm
you mean fatty acid, which has 80 carbons? Whau!

Well, I think you would get NO energy from that, because any enzyme wouldn't be able to digest that Anyway, what you probably want and need to hear is that you run 39-times the beta-oxidation (you have 80 carbons /2 = 40, but the last acetyl-CoA is released already in 39. cycle), so calculate, how many NADHs and FADH2s will you get And subsequently you have 40 acetyl-CoAs which go to TCA cycle and are degraded to CO2 and H2O
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

ayi
Garter
Posts: 2
Joined: Mon Oct 04, 2010 3:22 am
Can you totally break it down for me? Sorry, I am not a microbiology major and it doesn't explain it very well in my text book.

JackBean
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm
OK, well, the fatty acids are broken down in beta-oxidation
http://en.wikipedia.org/wiki/Beta_oxidation

in this process, you always get off 2 carbons in the form of acetyl-CoA, so after one cycle you will have 78 carbons, after 2 cycles it's 76 carbons, after 3 cycles 74 carbons etc. etc. up to 38th cycle you get 4 carbons, which are split appart in 39th cycle (there's some calculation also on the Wiki). So, you end up with 39 cycles and 40 acetyl-CoAs.
In each cycle you get 1 NADH and 1 FADH2, each giving let's say 2.5 and 1.5 ATP respectively. So you have 39 NADHs and 39 FADH2s, so calculate the ATPs.
Well, the 40 acetyl-CoAs enter the TCA cycle
http://en.wikipedia.org/wiki/Citric_acid_cycle
in one cycle you get 1 ATP (equivalent of GTP), 3 NADHs, one FADH2 and 2 CO2
http://en.wikipedia.org/wiki/Citric_acid_cycle#Products
so, you end up with 40 ATPs, 120 NADHs, 40 FADH2 and you don't care about the CO2 (otherwise 80;).
Now just calculate, how many ATPs you get and sum with the previous ones
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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