Rate of Metabolism

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Nicolodn
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Rate of Metabolism

Post by Nicolodn » Sun Oct 03, 2010 3:35 am

If 'A' takes a longer time to reach it's maximum growth, and 'B' takes a shorter time to reach it's max growth...but they have different Max. population....
that still means that 'A' has a slower metabolism?

Also, is there any reason why Grande strain of yeast would under the same conditions of no oxygen as the Petite strain of yeast have higher cell yield? Or is that just an experimental thing?

THanks.

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JackBean
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Post by JackBean » Sun Oct 03, 2010 10:17 am

you need to measure the rate of metabolism, e.g. by use of either radiolabeled or heavy atoms labeled compounds. However, you should measure something, what is common to both strain, like if you're missing the mitochondria in one strain, you probably won't see any CO2 production, although the glucose could be theoretically consumed faster.

Anyway, if you want to measure it by the measurement of OD600, you need to find time point, when either batch is saturated yet
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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