## Simple C1 V1 question

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

shanker
Garter
Posts: 1
Joined: Sat Oct 02, 2010 2:09 am

### Simple C1 V1 question

Advance apologies for such a basic question. I think I am correct, but want to dbl check....

I have a bacterial culture that I did a 10:1 dilution (100ul culture into 900ul 10mM MgSO4, blank = 100ul 2YT into 900ul 10mM MgSO4) ), read it on a spec and got .466 OD @600nm. I need 35 mls of the culture. I have to resuspend _at_ 1.0 OD. I need to figure the volume of buffer to resuspend after spinning down the 35 mls of culture.

C1 = 4.66 OD
V1 = 35 mls

C2 = 1.0 OD
V2 = ??

I calculated V2 to be 163.1 mls. Is this correct?

JackBean
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm
how much is 35*4.66? Yes, it is 163.1

BTW the units do not have plural
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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