Number of cells on Chamber slide

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convertin
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Number of cells on Chamber slide

Post by convertin » Thu Aug 04, 2005 3:26 pm

Hello!
I have a basic question. How many cells should I plate on a 4 well chamber slide (area=1.8 cm sq.). I need to do immunofluorescence after the overnight plating. So don't want them too confluent or too sparse. I know it depends on the cells I'm plating, but can someone give me a rough idea!

Thanks in advance!

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