non fat milk in blocking

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michelragno
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non fat milk in blocking

Post by michelragno » Fri Aug 20, 2010 9:18 am

Hello,

Why we must use the non fat milk ( and no the fat milk) for membrane blocking ?
What is the effect of lipids (of the fat milk) on immunodetection ?

Thanks for informations

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canalon
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Post by canalon » Mon Aug 23, 2010 10:27 pm

Not sure if there are any effects, although coating your membrane with fat might be a problem to the following steps. And I guess that this is the main point, non fat milk is as cheap and easily accessible than milk, and by using it you have basically what you want: a cheap protein suspension. Trying with the fat version might not change the results, but it could, so why even bother.
Patrick

Science has proof without any certainty. Creationists have certainty without
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