Hot start pcr
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Hot start pcr
hello every body,
I am in intense need of the protocol for hot start pcr, if any one knows all steps , please write here or give the reference
thanks in advance
zia
I am in intense need of the protocol for hot start pcr, if any one knows all steps , please write here or give the reference
thanks in advance
zia
If you do not have a special hot start polymerase (in wax beads, attached to antibodies or any other technique) you simply add a pause after the initial denaturation at the first step of your PCR and add the polymerase manually at that moment and then start the cycling as usual. Nothing very complicated.
Patrick
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)
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