cy3 and cy5-dCTP labelling
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cy3 and cy5-dCTP labelling
I purified total RNA from two different cell lines stably expressing an empty vector (as a control) and vector having two inserts. Now, I want to perform microarray. We have Cy3-dCTP and CY5-dCTP. Some protocols want you to purify mRNA and reverse transcribe it to cDNA, the others use total RNA to make cDNA. Which one is more suitable for these dyes? Is anyone using these dyes for cDNA or RNA labelling? I need a good protocol for it.
Thanks in advance..
Burcu
Thanks in advance..
Burcu
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