tyrosine solution

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piefke
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tyrosine solution

Post by piefke » Fri May 21, 2010 9:42 am

Hi

I want to do an enzyme assay in EPPS buffer pH= 7. I want to test the effect of tyrosine on the activity of my enzyme and therefore I have to add tyrosine in different concentrations to the buffer (end concentration
in 100 µl assay 10 - 250 µM).
The problem is: Tyrosine is not very soluble in water or in the buffer. I can´t solve it in HCl because my assay conditions then get acidic. Do you have any idea or experiences in working with amino acids?

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JackBean
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Post by JackBean » Fri May 21, 2010 10:17 am

what is the solubility of Tyr in water? You could make stock solutions in DMSO. I think, that 250 uM should be soluble, shouldn't it?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

piefke
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Post by piefke » Fri May 21, 2010 10:45 am

But don´t you think DMSO will disturb the activity of my enzyme?

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JackBean
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Post by JackBean » Fri May 21, 2010 12:36 pm

that depends on how much will you put in of course ;) We're typically adding 10 ul of substrate into 500 ul reaction. If you measure spectoscopically, you have to measure against blank, which contains DMSO of course ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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