Microarray CGH

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sam2400
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Microarray CGH

Post by sam2400 » Thu May 20, 2010 3:45 pm

Does anybody know how the two different samples of DNA ( control & test ) are fluorescently labelled before being applied to a microarray chip.

I am assuming that the DNA is amplified by PCR in the presence of fluorescently labelled nucleotides but can find no evidence to back this up. Anybody know if this is correct, or can provide the correct method.
Thanks

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JackBean
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Post by JackBean » Thu Jun 03, 2010 8:28 am

Yeah, I think it's by labeled nucleotides. But you can use any label you like. I think, that some primers bound to avidin are used as well.
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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canalon
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Post by canalon » Fri Jun 04, 2010 2:21 am

Patrick

Science has proof without any certainty. Creationists have certainty without
any proof. (Ashley Montague)

fourpeng
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Post by fourpeng » Fri Jun 25, 2010 9:58 am

primers bound to avidin are used as well

That is normally applied in Genetic Diagnosis

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