Discussion of all aspects of cellular structure, physiology and communication.
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Hi, I am new to muscle cells culture. I am culturing embryonic skeletal muscle cells in vitro. Someone told me glutamate will only stimulate msucle fibers if co-cultured with neuron cells. But glutamate will not stimulate muscle fibers if there are no motor neuron cells. Is it true? Because I found irregular contractions when I added glutamate into single muscle cell culture. Any ideas? Thanks!
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