% light transmission to enzyme activity graph

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JTeighty
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% light transmission to enzyme activity graph

Post by JTeighty » Wed May 05, 2010 3:54 pm

Hi,

I have drawn a graph of percentage light transmission for an enzyme (postivite correlation) and I want to change this into a graph showing enzyme activity (should be a negative correlation). How can I do this? Would inversing the graph work?

Thanks

JT80

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JackBean
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Post by JackBean » Thu May 06, 2010 7:41 am

what are you doing? What is your graph?

I think, that you want to convert transmission to absorbance...
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

JTeighty
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Re:

Post by JTeighty » Thu May 06, 2010 1:27 pm

JackBean wrote:what are you doing? What is your graph?

I think, that you want to convert transmission to absorbance...


I'm measuring effect of acid concentrations on an enzyme. I used percentage transmission as gave better results. On x axis is concentration an y axis is % light transmission. I want to make y axis enzyme activity..

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JackBean
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Post by JackBean » Thu May 06, 2010 8:20 pm

so, on x-axis you have the concentration of acid, right?

You now need to know the extinction coefficient for your substance and wavelength and just convert transmission to absorbance ;)
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

JTeighty
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Re: % light transmission to enzyme activity graph

Post by JTeighty » Thu May 06, 2010 8:45 pm

Ah I see. Thanks a lot :) :)

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