Question about an Arabidopsis T-DNA insertion

Plants!

Moderators: honeev, Leonid, amiradm, BioTeam

Post Reply
highyms
Garter
Garter
Posts: 2
Joined: Wed Oct 19, 2005 4:54 am

Question about an Arabidopsis T-DNA insertion

Post by highyms » Mon May 03, 2010 2:10 am

Hi, colleagues,
We bought an an Arabidopsis T-DNA insertion line from SALK and found the seeds are all heterozygote of our interesting gene.
However, we find that the offsprings of this Arabidopsis T-DNA heterozygote line are all heterozygotes, no Wild-type and homozygotes?

Why?

Thnaks a lot,

Highyms

User avatar
JackBean
Inland Taipan
Inland Taipan
Posts: 5694
Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Mon May 03, 2010 6:35 am

what about lethality?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

Dong
Garter
Garter
Posts: 7
Joined: Wed Dec 23, 2009 10:34 am

Post by Dong » Wed May 05, 2010 12:04 pm

Have you check the flowers ?
the fertility

Post Reply

Who is online

Users browsing this forum: Google Adsense [Bot] and 1 guest