ELISA

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dam13
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ELISA

Post by dam13 » Thu Apr 29, 2010 10:27 pm

so we just did an ELISA test (classical and sandwich in 2weeks) and he asked a question that i cant figure out for the life of me. In the wells he said in order to get a good rxn and color change the antigen to antibody ratio has to be good or else you wont get a good color change, my question is why? why does this ratio need to be observed in order for a good color change to happen? any help would be awesome as lab finals are coming up!

Jillo725
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Post by Jillo725 » Fri Apr 30, 2010 1:43 am

Would it be that
if you have too much antibody they would block each other from binding to the antigen? which is why you don't wanna just shove a whole bunch of antibody on the antigen?
"There is no adequate defense, except stupidity, against the impact of a new idea."
— Percy Williams Bridgman, US physicist

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