Splitting frozen plant material for representative sampling

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Eral
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Splitting frozen plant material for representative sampling

Post by Eral » Mon Apr 26, 2010 3:01 pm

Hi,

I'm looking for hints and tips how to obtain representative samples when splitting frozen plant material. I was recently introduced to the concept of grab sampling which introduces bias in the sample removed from the homogenized tissue.

In my case I'm working with liquid nitrogen frozen plant material frozen in bulk. I need to split the material into several samples, including a sample to isolate RNA from. This sample should be between 100ug and 0.5 g. The most common practice in this case is to use a spoon/scope to remove the sample. This would be a typical example of grab sampling which has been shown to introduce a sample bias which could be up to 20% (http://www.quantachrome.com/technologie ... pling.html#goodsampling). That could have a large effect on a subsequent transcriptome analysis.

My problem is now to find a sample splitter or riffler which can handle frozen ground samples since thawing of the samples must be avoided when preparing RNA. The device would have to be chilled and the amounts should be suitable. Anyone with experience of this kind of sample splitting for representative sampling??

Many thanks,
Eral

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JackBean
Inland Taipan
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Joined: Mon Sep 14, 2009 7:12 pm

Post by JackBean » Tue May 04, 2010 1:40 pm

Hi,
there's no need to double your posts.

Do you really think, that there can be such big differences?
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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