micromolar conversion

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roniadam
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micromolar conversion

Post by roniadam » Thu Apr 22, 2010 6:19 am

Hi All

I am getting stuck

My primer concentration is 0.1 uM in 10 uL and I need to get 125 ng of this primer.
how many uL should i take from my primer in total reaction of 50 uL.

Is there any online calculator that can help me ???

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JackBean
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Post by JackBean » Thu Apr 22, 2010 8:01 am

calculator? :lol:

These are simple chemical calculations, I guess you should know them, if you're doing PCR.

m = n.M
c = n/V
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

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jonmoulton
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Re: micromolar conversion

Post by jonmoulton » Thu Apr 22, 2010 4:13 pm

You have a concentration in micromoles. You want to deliver a particular number of nanograms. First convert micromolar (which is micromoles/liter) to nanograms/liter. To do that you will need the molecular mass of your primer. Watch out for the prefixes, it is easy to mess up the calculations by incorrectly converting micro to nano. Once you have converted micromolar to nanograms/liter, determine how many microliters you need to get your desired number of nanograms.

roniadam
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Post by roniadam » Sat Apr 24, 2010 2:35 am

Hi
Thank you very much for your kine help

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