Integrating plasmid to Yeast

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molecule
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Integrating plasmid to Yeast

Post by molecule » Thu Apr 22, 2010 5:07 am

Hi, I;m not familiar with this technique and was wondering if someone can help out. I have a plasmid that has an ARS and my gene of interest and I need put this gene of interest in to an yeast strain to study it. Does anyone know how I can do that. More details about the technique will be very helpful. I tried to read about some yeast integration it was hard to comprehend.
thanks

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JackBean
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Post by JackBean » Thu Apr 22, 2010 7:51 am

what yeast do you use? I have used pPICZ and pGAPZ in Pichia, there was part of one endogenous genes, the plasmid was cut in one place and incorporated by homologous recommbination
http://www.biolib.cz/en/main/

Cis or trans? That's what matters.

molecule
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Post by molecule » Fri Apr 23, 2010 12:32 am

I'm looking at Fission yeast. Its the procedeur as to how to do that is what I;m no familiar with. thanks

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