What stain to use on a protozoan?

About microscopic forms of life, including Bacteria, Archea, protozoans, algae and fungi. Topics relating to viruses, viroids and prions also belong here.

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blwibwib
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What stain to use on a protozoan?

Post by blwibwib » Wed Jul 27, 2005 2:00 pm

I am currently doing a project looking at bumblebee parasites and infections, and one of these is a protozoan called Crithidia bombi. In order to look at it under a microscope I need to be able to stain it, and I have no idea what chemical to use, and neither does my supervisor. Does anyone have any suggestions?

Thanks!

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MrMistery
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Post by MrMistery » Wed Jul 27, 2005 6:57 pm

You need to know what exactly do you want to stain in the protozoan. You could use metil blue for coloring DNA but since the host cells also has DNA it won't do you much good...
By the way, usually protozoans are not stained... They can be viewd at an optical microscope fine... Try it. If you can't see anyhting, then try finding something to stain at the protozoan
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Post by blwibwib » Wed Jul 27, 2005 9:00 pm

Basically at this stage all I want to see is if there are protozoans present or not. Would I be able to see that without staining?

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Post by Dr.Stein » Thu Jul 28, 2005 1:27 am

Don't put methylene blue or strong dyes if you are not sure, it will destroy your object. Try to stain with supravital dyes, it is harmless and keeps your specimen alive for few times out of the body, but you must work fast for this. This is very easy. Simply just do like this: put the dye on your preparation, examine it under microscope that is connected to photomicrograph equipment. When you find your object, you can take its picture very soon for documentary thing. Good luck!
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Post by blwibwib » Thu Jul 28, 2005 9:00 am

Thank you very much, that's given me a lot to work with!
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MrMistery
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Post by MrMistery » Wed Aug 10, 2005 8:37 pm

By the way, many protozoans CAN be seen without dye
"As a biologist, I firmly believe that when you're dead, you're dead. Except for what you live behind in history. That's the only afterlife" - J. Craig Venter

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